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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in lots of tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two added splice variants had been described but thorough nucleotide sequence facts will not be nonetheless offered.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide usually consists of the epitope identified by the antibody.
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This information outlines more probable optimization of cellular and immunoassay parameters to ensure the absolute best results are...
Antibodies more info certain to the blocking peptide not bind into the epitope within the focus on protein. By evaluating the staining from your blocked antibody versus the antibody on your own, you can see which staining is unique.
Target Data Cyclin E belongs into the extremely conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation patterns which add into the temporal coordination of each and every mitotic party. Cyclin E sorts a fancy with and capabilities as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is particularly degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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